Microbiology gram negative- unknown project. | microbiology | California Community Colleges System Office

Sep 24, 2023

My investigation is on Gram-Negative. I will be attaching the descriptive chart

You do not need to fill anything in on the descriptive chart, there will be some blanks and that’s okay.
So the next thing you look at for the gram negative rod will be the oxygen requirement on your descriptive chart. If it is aerobic then you will need to move onto the species ID chart to compare Pseudomonas, Acinetobacter, and Alcaligenes. If its a facultative anaerobe then you keep going on the flow chart looking at motility. If its non-motile you would have Klebsiella or Shigella.

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If its motile you will have one of the many bacteria in Group IV. To distinguish group IV you go to the other Gram negative flow chart for Group IV and V to narrow it down. Be sure to pay attention to the variable results for citrate, that I mention in the zoom. Once you get to an end point on that flow chart you move to the species ID chart. Morphological, Cultural, and  Biochemical characteristics indicated are on the descriptive chart that will be attached.

The combination of all the results given will allow for the identification  of the bacterium to the Genus and Species level using flow charts and a species identification chart.   Followed by utilization of DNA sequencing to provide final confirmation of the genus and species. This paper will include the methodology used in the research, the results of your research, and interpretations and conclusions drawn from these results. Your conclusions must include the identification of your assigned bacterium down to the species level.

Suggested order to complete the report:
1.  Create your Table: The best place to start is create your Table of essential and non-essential
tests.  You should jot down the essential tests as you run through the identification using the
flow chart(s) and the species ID chart.  Any test that you had to look at the result of on the flow
chart and species ID chart is an essential test.

2.  Create your Dichotomous key/Flow chart:  Once the table is created the essential tests in the
table are the same tests that should go on to the flow chart, so this would be a good next step in
writing your paper.  Also you should do this soon after you have ID’d using the flow chart and
species ID chart that way it is fresh in your mind and will be easier to create. Even if you just
rough draft sketch it out on paper then you can later create the polished version in the

3. Write your Results section and complete your Results Journal: These can be completed in
either order or done simultaneously.  You will need to refer back to the Biochemical testing labs
to get the info such as appearance of the results and meaning of the results.  Remember for
your journal you will images from the internet and include the link to the webpage you found
the image on.  

4. Discussion:  Write your discussion.

5. Introduction: Write your introduction.  It may seem weird, but it kind of makes sense to write
your Introduction last, what better way to be able to introduce and summarize the project then
after you have written the entire report.  

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